RNA-seq analysis of in vivo region-specific PDX (G144) tumour cell populations and in vitro SOX10 overexpression in G144 cells

For in vivo experiments GFP+ G144 human GBM cells were injected in the caudoputament of CD-1 nude mice. After tumour formation, GFP+ G144 cells were acultely purified from microdissected mouse brain regions (tumour bulk, corpus callosum, striatum) and sequenced to determine gene expression profiles of the different invasive populations. In vitro G144 cells engineered to express inducible SOX10 vector were exposed to Doxycyline for two days and sequenced to determine gene expression profiles following SOX10 overexpression. 3 independent biological repeats were sequenced for analysis