A single cell atlas of the human liver tumor microenvironment

We identify the specific contribution of each cell type to the human liver tumor microenvironment and reconstructed hepatocytes zonation of the human liver using a combined approach of single-cell RNA sequencing and laser capture microdissection. Our work constitutes an important resource for identifying potential sources of vulnerabilities in liver tumors and metastases. Overall design: We analyzed six patients who underwent liver resection (colorectal metastasis, primary cholangiocarcinoma, and benign liver cyst). Two samples were extracted from each (malignant and non-malignant). All samples underwent single cells RNA sequencing using MARSseq. In parallel, frozen sections were prepared from each sample. Isolated specific zones of each sections was extracted using laser capture microdissection (LCM) and subjected to bulk sequencing using mcSCRBseq protocol.