Stopped-flow analysis of GroEL D398A-RW.

A. Analysis of fluorescence intensity changes upon addition of 1black trace indicates GroEL R231W, and the gray trace indicates D398A-RW. B. As in A, but with the duration of observation set to 100 sec. Gray trace indicates D398A-RW with ATP added (as in A), red trace indicates fluorescence changes upon addition of 1 mM AMP-PNP.

A. 对加入1black追踪剂后荧光强度的变化进行分析，其中黑色轨迹表示GroEL R231W，灰色轨迹表示D398A-RW。B. 类似于A，但将观察时长设置为100秒。灰色轨迹表示加入ATP后的D398A-RW（如同A中的情况），红色轨迹表示加入1 mM AMP-PNP后荧光强度的变化。