Electron Tomography of Inner Ear Ribbon Synapses

This dataset contains room-temperature electron tomograms of murine and rat inner ear ribbon synapses.It was used for training and evaluation of ribbon synapse structure segmentation in [1]. The data contains 6 tomograms from mice that were initially published in two different studies [2, 3], as well as 3 additional tomograms that were acquired following the same protocol as [3] but that were not included in that publication.It contains 19 tomograms from rats from [4].The data is organized into three different subfolders "rat", "tether" and "vesicle_pools", containing tomograms and annotations stored as hdf5 files.Each hdf5 file contains the following internal datasets:- raw: The tomogram data.- labels/PD: Annotations for the presynaptic density, annotated with IMOD and exported to a binary mask.- labels/PSD: Annotations for the postsynaptic density, annotated with IMOD and exported to a binary mask.- labels/membrane: Annotations for the membrane of the active zone, annotated with IMOD and exported to a binary mask.- labels/ribbon: Annotations for the synaptic ribbon, annotated with IMOD and exported to a binary mask.- labels/vesicles: Annotations for the vesicles close to the active zone. Annotated with IMOD and exported to instance masks.

[1] Muth, Moschref et al., 2024, Preprint to be published[2] Chakrabarti et al. Vesicle sub‐pool organization at inner hair cell ribbon synapses, EMBO reports, 2018, DOI: 10.15252/embr.201744937[3] Chakrabarti et al., Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses, eLife, 2022, DOI: 10.7554/eLife.79494[4] Michanski, Kapoor et al., Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing, EMBO press, 2023, DOI: 10.15252/embr.202256702