Gene profiling using microarray analysis between the ΔMet treated and control groups at different stages

Undifferentiated cells, hiPSC-derived differentiated cells on day3 DE cells, day 5 PG cells, day 11 PP cells and day 25 EC cells were compared, using GeneChip™ Human Gene 2.0 ST Array Methionine deprivation (ΔMet) pretreatment potentiates late-stage differentiation of human iPS cells into insulin-expressing β cells Cells were harvested at undifferentiated or differentiated stages for RNA extraction and hybridization on Affymetrix microarrays. We performed gene expression profiles of hiPSCS derived pancreatic cells cultured in complete (comple) or Met-deprived conditions (deltaMet) for 5h before differentiation. And human islte were used as a comparison