Organotypic stromal cells impact endothelial cell transcriptome in 3D microvessel networks

ECFC-ECs are considered to be a plastic and naive endothelial cell. We combined ECFC-ECs with different stromal cells to see the resultant change of endothelial cell transcriptome due to different stromal cells. ECFC-ECs and a variety of organotypic stromal cells (Bone marrow, heart, skin, lung, pancreatic cancer, normal pancreas) were combined in 3D in vitro fibrin hydrogels and allowed to grow for 7 days into microvessel networks.