Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all. Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all

Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. GISH) and mc-FISH analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D.EST-STS marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line, and that the P. huashania 2Ns chromosome shares high sequence similarity to wheat chromosome 2D. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding. Overall design: To further clarify the chromosome composition of H139, the Wheat Axiom 660K Genotyping Array was used for genotyping. A total of one sample (H139) was genotyped by wheat Axiom 660K Genotyping Array, which is due to the data of one sample enough to revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line.