Spatial total RNA sequencing of formalin-fixed paraffin embedded tissue by Spatial Random-seq

Current spatial transcriptomic methods have been widely used to resolve gene expression; however, these methods are limited to fresh or fresh-frozen samples due to unsuccess of oligo(dT) primers in degraded RNA samples. Here we develop a spatial random-sequencing (spRandom-seq) technology for Formalin-fixed paraffin-embedded (FFPE) tissues by capturing full-length total RNAs with random primers. This approach provides a powerful spatial platform for clinical FFPE specimens and promises enormous applications in biomedicine. Overall design: Spatial single cell RNA-sequencing of FFPE mouse tissues using spRandom-seq , benchmark analysis was performed.