Adenosine to Inosine RNA editing level in mouse lungs from Adar1 K999N mutants or hypoxia-exposed mice

Hypoxia is a PH trigger which significantly downregulates ADAR1 and reduces A-to-(I)G editing in mouse lungs. Mice have Adar1 misssense mutation K999N were used to determine specific ADAR1-specific RNA editing sites in lungs. We performed RNA sequencing on the lung tissues isolated from mice carrying Adar1 K999N mutations and exposed to 3 week hypoxia. Overall design: To investigate the effect of hypoxia on ADAR1-mediated RNA editing in mouse lungs, we treated wildtype or Adar1 K999N mice with hypoxia (10% oxygen) exposure for 3 weeks. RNA sequencing was performed and the overall A-to-I(G) RNA editing level was analyzed.