Unmasking of Protein Phosphatase 2 Regulatory Subunit B as a crucial factor in the development and progression of dilated cardiomyopathy

The PPP2R5D encodes for a Protein phosphatase 2A (PP2A), one of the four major Ser/Thr phosphatases negatively controlling cell growth and division. PP2A is consisting of a common heteromeric core enzyme with a catalytic subunit and a B regulatory subunit that might control the substrate selectivity and catalytic activity. We investigated the potential role of Ppp2r5d for the cardiomyocyte (cultured mouse cardiomyocytes). The interfering RNA against Ppp2r5d (Case) or the negative control siRNA (Control) were transfected into MCMs（mouse cardiomyocytes） with Lipofectamine™ RNAiMAX transfection reagent. Forty-eight hours after transfection, the medium was changed to glucose-free media. The samples were then collected for a microarray gene expression study after overnight incubation. One sample for each group.