HIV-1 Bal and SIV CP-MAC gp120 purified from virions lack O-linked carbohydrate.

Electrospray Nano-LC-MS/MS summary showing the lack of O-glycan occupancy of the conserved Thr of HIV-1 Bal gp120 and SIV CP-MAC gp120 when protein was purified from virions. Also shown is the lack of O-glycan occupancy on recombinant secreted gp120 of the T499S mutant of HIV-1 strain NL4-3. m/z is mass to charge ratio. MW is molecular weight. HexNAc.Hex is core 1. HexNAc.Hex.NeuAc is monosialylated core1. HexNAc.Hex.NeuAc2 is disialylated core 1. HexNAc2.Hex2.NeuAc is core 2.HIV-1 Bal and SIV CP-MAC gp120 purified from virions lack O-linked carbohydrate.