SIP exp - Glucose amendment of peat catotelm

Deep peatlands store vast quantities of sequestered carbon, but microbial decomposition is limited by energy availability and transport. Climate change, with increased root growth, may destabilize this vast store. Understanding microbial responses to changing plant inputs is crucial for predicting carbon release. Our study used 13C-labeled glucose to investigate deep peat carbon degradation mechanisms (>1 m). Samples were collected at the SPRUCE site incubated with 13C-labeled glucose, and the diversity and composition of prokaryotic communities were determined by applying a high-throughput sequencing-based protocol that targets PCR-generated amplicons from V4 variable regions of the 16S rRNA gene using the bacterial primer set 515F and 806R after SIP fractionation.