Effective angular diffusion coefficients DMT of astral microtubules.

DMT was calculated separately for the microtubules extending from the SPB destined to the daughter cell and from the SPB destined to the mother cell, in each of the 3 strains (wild type, kar9Δ, num1Δ). DMT was obtained by fitting the equation MSAD = 2DMTΔt+offset to the mean squared angular displacement (MSAD) data shown in Figure 2, with weights 1/s.e.m. The errors are the square root of the diagonal elements of the covariance matrix from the linear fit, n denotes the number of microtubules. The number of cells was 9 for wild type, 10 for kar9Δ and 10 for num1Δ.