Expression profiling of lncRNAs, miRNAs and mRNAs and their differential expression in leiomyoma using next generation RNA sequencing

Total RNA was isolated from leiomyoma and paired myometrium (N=8) and samples from three pairs were subjected to RNA sequencing. After analysis, 5941 lncRNAs (2813 up- and 3128 down-regulated at =1.5 fold), 148 miRNAs (56 up- and 96 down-regulated at =1.5 fold) and 3855 mRNAs (2030 up- and 1855 down-regulated at =1.5 fold) were differentially expressed in leiomyomas. Using QRT-PCR we further confirmed the expression of HULC, lnc-MEG3, LINC00890, TSIX, LINC00473, lnc-KLF9-1 and lnc-POTEM-3 (lncRNA-ATB) in leiomyoma and matched myometrium (N=8). Our results presented here provide a comprehensive expression profile of lncRNAs in leiomyomas with concurrent integrated expression of miRNAs and mRNAs in leiomyomas. Overall design: Identify the expression profile of lncRNAs with concurrent assessment of miRNAs and mRNAs profiles in leiomyomas and paired myometrium using next generation RNA sequencing