Binding of the anti-mycolactone mAbs to different reporter molecules.
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https://figshare.com/articles/dataset/Binding_of_the_anti-mycolactone_mAbs_to_different_reporter_molecules_/12575672
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NeutrAvidin-coated plates were coated with the different reporter molecules at 1 μg/ml and 3-fold dilution series of the different mAbs starting from 5 μg/ml were added and allowed to bind. Bound mAbs were detected with an HRP-conjugated secondary antibody and TMB. Binding was graded based on the absorbance (OD) values measured at 450 nm as follows: (-) OD < 0.5, (+) OD 0.5–1, (++) OD >1–1.5, (+++) OD > 1.5.
创建时间:
2020-06-26



