Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [scRNA-seq]

To establish an in vitro endometrial co-culture model, endometrial organoid was co-cultured with endometrial stromal cells in a 3D hydrogel matrix. During this co-culture process, tubular gland development was identified from endometrial organoids, which showed the molecular, morphological and functional properties of human endometrial glands. To further characterize the genetic drive for this phenomenon, we conducted single-cell RNA sequencing analysis at day 1 and day 9 after co-culture. Three-dimensional co-culture models were collected separately at day 1 and day 9 after co-culture, digested into single cells and send for single-cell RNA sequencing analysis to identify the regulatory genes.