RNASeq of Circadian clock genes form wild type and Bmal1 knockout mice of airway epithelial cells

Circadian time-keeping in mammals is regulated by transcription/translation feedback oscillator loops driven by a set of dedicated clock proteins. A significant proportion of the expressed transcriptome is regulated in a circadian manner in multiple body organs and cell types, with reports of up to 43 percent of protein-coding gene transcripts oscillating in at least one organ. The current experiment is designed to demonstrate the important role of Bmal1 and circadian clock in pulmonary physiology and disease. In this experiment RNA is either extracted from the mice lung tissues (by a laser capture procedure) or extracted from cultured airway tissue cells. The protein coding RNAs were then sequenced with Illumina HiSeq technology.