Visceral adipose tissue macrophage subsets. Visceral adipose tissue macrophage subsets

We identified two different subsets of macrophages in visceral adipose tissue. These two subsets were transcriptomically different. We observed that C3CR1-low CCR2-low macrophages are transcriptomically different after myocardial infarction (MI). Overall design: We sorted CX3CR1-high CCR2-high and CX3CR1-low and CCR2-low macrophages from visceral adipose tissue and blood monocytes from mice in the steady state, and CX3CR1-low and CCR2-low macrophages from visceral adipose tissue of mice with MI or sham surgery directly in RNA extraction buffer (Applied Biosystems Pico Pure Kit for mRNA extraction) and performed RNA-seq analysis.