Transcriptional characterization of PD-1 positive and negative Foxp3 negative CD4 positive T cells from liver of 5- and 10-day-old Aire-deficient C57BL/6 mice. Transcriptional characterization of PD-1 positive and negative Foxp3 negative CD4 positive T cells from liver of 5- and 10-day-old Aire-deficient C57BL/6 mice

The first T cells to arrive in the liver were mostly T regulatory (Treg) cells and metabolically active, highly proliferative T conventional (Tconv) cells. The Tconv cells had unusually high expression of PD-1 and the IL-33 receptor, ST2. As these PD-1+ Tconv cells accumulated in the tissue, they gradually lost their expression of ST2, ceased to proliferate and acquired an anergic phenotype. Overall design: Gene expression profiles of flow cytometry sorted DAPI negative CD45 positive TCRb positive CD4 positive Foxp3 negative cells from liver of 5- and 10-day-old B6.Aire-KO mice