Oncogenic HOXB8 is driven by MYC-regulated super-enhancer and potentiates colorectal cancer invasiveness via BACH1 [RNA-seq]

HOXB8 acts as a transcription facor, thus we knock down HOXB8 gene by doxycycline induced shRNA in colon cancer cells and profiled the change of transcriptome after knocking down HOXB8 Overall design: Two independent shRNAs targeting HOXB8 were stably transduced into HCT116 cells. The expression of these shRNAs is induced by the presence of doxycycline (dox). Therefore, the control group includes two independent repeats of two shRNAs respectively, while the HOXB8 knock-down group includes two independent repeats of two shRNAs treated with doxycline respectively.