HLY-08-02 Lipofuscin and Euphausiid Aging

This dataset pertains to age determination in crustaceans relies on quantification of a fluorescent "age pigment," lipofuscin, that accumulates in eye stalks of krill over time. Lipofuscin includes a mixture of protein oxidation products formed in neural tissues. The extracted products are quantified using a fluorescence detector. The concentration of lipofuscin is normalized to the protein content from the same tissues. The ratio of lipofuscin to protein concentration is the Lipofuscin Index and is calculated for each individual organisms. In order for the index to reflect chronological age, a calibration is required using euphausiids of known age. A comparison of the calculated ages to the specific lengths for each krill provides the ages for those krill analyzed during each cruise. These data are preliminary until an age calibration curve is completed following rearing. These data were collected aboard the United States Coast Guard Icebreaker Healy. Data were collected during the Bering Sea Ecosystem Study-Bering Sea Integrated Ecosystem Research Program (BEST-BSIERP). BEST-BSIERP together are the Bering Sea project.

本数据集涉及甲壳类动物年龄的测定，其基于对积累在磷虾眼柄中随时间累积的荧光‘年龄色素’——脂褐素——的量化。脂褐素包含在神经组织中形成的蛋白质氧化产物混合物。通过荧光检测器对提取的产物进行量化。脂褐素的浓度被标准化为相同组织中的蛋白质含量。脂褐素与蛋白质浓度的比值即为脂褐素指数，并对每个个体生物进行计算。为了使该指数反映生物的实际年龄，需要使用已知年龄的桡足类动物进行校准。通过将计算出的年龄与每个磷虾的具体长度进行比较，得到在每次巡航期间分析的磷虾的年龄。这些数据在完成年龄校准曲线之前为初步数据。这些数据是在美国海岸警卫队冰breaker Healy 号上收集的。数据收集于白令海生态系统研究-白令海综合生态系统研究计划（BEST-BSIERP）期间。BEST-BSIERP 合称为白令海项目。