Gene expression of human corneal epithelial cells grown with irradiated human fibroblasts or irradiated mouse 3T3 feeder layer .

human corneal epithelial cells were isolated from healthy human donor eyes. Cells were cultured with irradiated 3T3 (i3T3) murine fibroblast feeder cells, irradiated human corneal fibroblasts (iHFL) or without feeder layer Three human corneal epithelial cells populations (primary cells) were cultured in three conditions (without feeder layer, +iHFL or +i3T3) and analyzed by gene profiling.