Dispersal, habitat filtering, and eco-evolutionary dynamics as drivers of local and global wetland viral biogeography

Wetlands store 20–30% of the world's soil carbon, and identifying the microbial controls on these carbon reserves is essential to predicting feedbacks to climate change. Although viral infections likely play important roles in wetland ecosystem dynamics, we lack a basic understanding of wetland viral ecology. Here 63 viral size-fraction metagenomes (viromes) and paired total metagenomes were generated from three time points in 2021 at seven fresh- and saltwater wetlands in the California Bodega Marine Reserve. We recovered 12,826 viral population genomic sequences (vOTUs), 4.4% of which were also detected at the same field site two years prior, indicating a small degree of population stability or recurrence. Viral communities differed most significantly across the seven wetland sites and were also structured by habitat (plant community composition and salinity). Read mapping to a new version of our reference database, PIGEONv2.0 (now with 515,763 vOTUs), revealed 196 vOTUs present over ..., , This zip package includes: 
1) All vOTUs from the Bodega Bay experiment, predicted from viromes, recovered in one or multiple samples.
2) All vOTUs in the Phages and Integrated Genomes Encapsidated Or Not v2.0 (PIGEONv2.0) database. 
3) A table with habitat origins for each of the vOTUs in the PIGEON database and the publication the contig came from.
4) MAGs from the Bodega Bay experiment total soil metagenomes.