16S rRNA gene sequencing of faecal, urine and vaginal samples from women with Lichen Sclerosus

The study aimed to evaluate and compare the composition of the urinary, vaginal and gut microbiota in women with LS versus non-affected controls.Women diagnosed with LS (n=16) and matched controls (n=14) were enrolled in the study. From each participant, midstream urine, upper and lower vaginal swabs as well as faecal samples were collected. The microbiota composition was assessed using 16S ribosomal RNA (rRNA) gene sequencing of the V4 hypervariable region.