Cell-specific protein expression in Alzheimer's disease prefrontal cortex

Analyzing the proteomes of different brain cell types is fundamental for understanding the pathophysiology of Alzheimer's disease (AD). However, spatial analysis of these diverse and limited cell populations poses significant challenges. The GeoMx Digital Spatial Profiler (DSP) platform analyzed protein levels in the prefrontal cortex of AD and non-AD brains. The platform interrogated 76 proteins and used immunofluorescence to distinguish between three cell types. Neprilysin, which promotes Aβ degradation, was significantly higher in AD neurons and microglia. LAMP2A level was higher in neurons of individuals with AD compared to a control group. Additionally, markers of neuroinflammation, such as CD11c, CD11b, and CD163, were also elevated in AD neurons. Our findings indicate that the DSP platform effectively facilitates cell-specific snapshots of the AD brain proteome., , # Cell-specific protein expression in Alzheimer's disease prefrontal cortex

**Dataset DOI:**
[https://doi.org/10.5061/dryad.w6m905r12](https://doi.org/10.5061/dryad.w6m905r12)

#### GENERAL INFORMATION

**1. Study Objective:**
This dataset supports a spatial proteomics study investigating cell-specific alterations in protein expression within the prefrontal cortex (Brodmann area 8/9) in Alzheimer's disease (AD). The analysis aims to characterize how protein signatures differ between AD and control samples at the cellular level, focusing on neurons, astrocytes, and microglia.

**2.Authors Information:**

* Principal Investigator Contact Information

Name: Mohammad Haeri, MD PhD

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Institution: University Of Kansas Medical Center
Email: [e-mail]
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* First Author Contact Information

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Name: Maryam Gholampour, PharmD
Institution: University Of Kansas Medical Center
Email: [e-mail]
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**3. Data Collection Dates:** 2022-2023 

**4. Geographic Locat..., The Kansas University Medical Center Human Subjects Committee (KUMC HSC) issued approval for all involvement of human subjects, and all participants provided informed consent prior to enrollment. This investigation was conducted in accordance with the Code of Ethics set forth by the World Medical Association (the Declaration of Helsinki).