Young and Aged Striatal Astrocyte MERFISH Panel

Given that the striatum is a large, multifunctional nucleus, we next aimed to understand where astrocyte subtypes exist in the striatum at a single-cell resolution using MERFISH. We reproducibly identified the seven astrocyte subtypes, some of which were dorsal (A1, A7), medial (A3, A6) or ventral dominant (A4). MERFISH was performed according to Vizgen's instructions. Data was initally processed using MERLIN, and cell segmentation completed with Vizgen software.