Genome wide RNA-sequencing of sorted human pluripotent stem-cell derived endothelial cells with CLDN5-GFP reporter (GFP+ and GFP-)

CLDN5-GFP reporter has been engineered in hPSCs. These cells have been differentiated to Ecs. Differentaited Ecs were sorted into GFP+ and GFP- corresponding to CLDN5+ and CLDN5- to study gene expression of high-barrier resistance. ECs differentiated from engineered hPSC carrying the CLDN5-GFP reporter (2 separate clones) were sorted with BD FACSAria III into GFP+ and GFP- populations (6 individual sorting procedures for each clone). RNA was isolated from all the samples and library generated that was sequenced using IIllumina Hiseq platform.