File S1 - A Novel miR-451a isomiR, Associated with Amelanotypic Phenotype, Acts as a Tumor Suppressor in Melanoma by Retarding Cell Migration and Invasion

Contains the following files: Figure S1. miRDeep2 output file showing miR-451a isomiRs in NS3 library. This example provided positions and read counts, showing that miRBase (v18) sequence was not the abundant isomiR; in fact, isomiR1 and isomiR2 were the most abundant sequences. Only the isomiRs with the highest read counts are shown. Figure S2. miRDeep2 output file showing miR-451a isomiRs in PCM5 library. Similar to NS3, miRBase (v18) sequence was not the most abundant; instead isomiR1 and isomiR2 were the most abundant sequences. Figure S3. Robust miR-451a.1 expression was detected in epidermal keratinocytes in normal skin. (a and b) The signal for miR-451a.1 (red) was readily detected in the nuclei and cytoplasm of epidermal keratinocytes of a normal skin specimen. (c) This signal was not detected in the dermal melanoma cells or the overlying keratinocytes. (d and e) Scramble controls showed no signal in any cell type in normal skin; or (f) invasive melanoma. (h) U6 signal was robustly detected in the nuclei of epidermal keratinocytes and dermal nevus cells; (i) but not in the scramble control. The dotted line represents epidermal-dermal junction. Images for miR-451a.1, scramble and U6 probes were acquired under the same constant parameters. The original magnification was 200X for A, D, H and I; 400X for B, C, E and G. Figure S4. miR-451a.1 was not detected in additional scramble controls. (a-c) The signal for miR-451a.1 (red) was not detected in nevus scramble controls or (d-f) melanomas scramble controls were negative. Tables S1-Table S6.
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