House fly and manure samples from dairy cattle farms Raw sequence reads

House flies thrived in confined cattle operations and cause nuisance to animals and vector a wide range of microbes that could have acquired from animal waste. However, our understanding of risk house flies poses in acquiring and disseminating of bacterial communities is still limited. Characterizing and evaluating the bacterial communities of individual house flies and their habitat substrate (manure) samples will allow us to understand the role of house flies as a vector of bacteria in dairy cattle operations. This study characterized bacterial communities of individual female house flies and manure samples collected during May to August from dairy farms located in Florida, North Carolina and Tennessee in the United States. Bacterial community composition in fly and manure samples reflect local farm environment. House fly shared all of bacterial taxa within manure samples (100% of total manure bacterial community abundance). Highly prevalent bacterial communities included potential pathogenic taxa of human and animals such as Corynebacterium, Staphylococcus, Streptococcus, Enterobacteriaceae, Providencia, Acinetobacter, and Clostridium. These results showed that house flies are important carrier of cattle pathogens and may contributes to disseminate those into farm animals.