GNPS - Root metabolome characteristics of different root functional types in response to water and N limitations

One upland and one lowland switchgrass genotypes were grown in native soil under a rainout shelter and for three years under well-watered and water limited conditions. Further, two N treatments, + 67.25 kg N/ha and 0 fertilizer N application, were superimposed on the water availability treatments. Each of the 8 treatments (2 genotypes x 2 water availability x 2 N availability) was replicated three times with each experimental unit consisting of six plants in each of the treatments. Root and soil samples were collected at the end of the third year and roots were separated into transport and uptake roots to study how the metabolite profile in the two switchgrass ecotypes is influenced by water and N availability. This analysis will be coupled with a classification and identification of the root associated as well as rhizosphere microbiome.

The work (proposal:https://doi.org/10.46936/10.25585/60001049) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.