Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons

This dataset covers mechanical and thermal sensitivity in PV-Cre rats (RRID:RRRC_00773) beginning in adolescence and continuing into adulthood. The rats were tested weekly for eight weeks. After the first test, half the rats began adolescent intermittent ethanol vapor exposure which continued for 4 weeks. Rats then underwent surgery to express channelrhodopsin in the BLA, and a viral cre-depedent mCherry tag in the prelimbic cortex. Retrobeads were injected in the ventrolateral periaqueductal gray. This allowed for slice electrophysiology recordings from prelimbic parvalbumin interneurons and prelimbic pyramidal neurons projecting to the ventrolateral periaqueductal gray. After recovering from the surgery, rats underwent a carrageenan inflammatory paw pain challenge. After assessing the effects of adolescent alcohol exposure in conjuction with the pain challenge on mechanical and thermal sensitivity, slice electrophysiology recordings were obtained from neurons in the prelimbic cortex. ..., , # Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons

Dataset DOI: [10.5061/dryad.wwpzgmswd](10.5061/dryad.wwpzgmswd)

## Description of the data and file structure

These data include both behavioral assessments of mechanical and thermal pain sensitivity as well as data from slice electrophysiology recordings measuring synaptic function at basolateral amygdala inputs onto prelimbic parvalbumin positive interneurons and pyramidal neurons projecting from the prelimbic cortex to the ventrolateral periaqueductal gray. For the behavioral assessments, the electronic Von Frey and Hargreaves tests were used. Data were collected weekly for 8 weeks beginning at postnatal day 24 (PD 24) and continuing until PD 80. Half the rats began alcohol vapor exposure at PD 28. Rats underwent final reflexive pain assessments at approximately PD 109. This involved a baseline pain assessment followed by a carrageenan or saline (control) injection into the hindpaw. The...,