Replication Data for: Lipid nanocarrier containing eugenol for Denture Hygiene

This study aimed to develop a lipid nanocarrier containing eugenol and evaluate its efficacy as a sanitizing agent for denture hygiene, targeting biofilms of Candida albicans and Candida glabrata. The formulation was prepared using the emulsification method followed by ultrasonication and characterized in terms of particle size, polydispersity index (PDI), zeta potential (ZP), and encapsulation efficiency (EE).

The experimental design included in vitro tests, where biofilms of Candida species were induced on polymethyl methacrylate resin specimens. The specimens were subjected to four treatments: nanocarrier containing eugenol (NFE), free eugenol (FE), saline solution (SS), and nanocarrier without eugenol (NFW). After eight hours of immersion, antifungal efficacy was assessed by counting colony-forming units (CFUs).

The results showed that NFE presented particles with an average size of 199.5 nm, high homogeneity (PDI 0.07), and an encapsulation efficiency of 83%. The formulation demonstrated significant fungicidal activity, reducing biofilms of both tested species, with minimum inhibitory concentration (MIC) values up to 10 times lower than those of free eugenol. Statistical analysis, conducted using ANOVA and Tukey’s test, confirmed the superiority of NFE compared to the other tested groups.

This study contributes to the development of new strategies for denture hygiene, highlighting the potential of lipid nanocarriers to optimize the antifungal action of natural compounds.