Raw dataset of light-sheet-microscopy images for a mouse brain sample labeled for Dorsal Raphe Nucleus serotonergic neurons

Comprehensive mapping of neuronal connections across entire nervous systems remains a fundamental challenge in neuroscience. Here, we introduce LINCS (Labeling Individual Neurons with Chemical dyes and controllable Sparseness), a technology that achieves rapid, ultrabright, and photostable labeling of specific cell types throughout the entire mouse brain and body. LINCS utilizes an engineered, solubility-enhanced biotin ligase for in vivo biotinylation, followed by rapid whole-mount staining with a high-affinity monovalent streptavidin. When integrated with tissue clearing and light-sheet microscopy, this system creates an efficient pipeline for profiling long-range neuronal projections across both the central and peripheral nervous systems. This is a raw dataset (tiff files) of a LINCS whole-brain sample (DRN serotonergic neurons) and its downsampled version (factor: 0.125). More detail is described in the README pdf file.