RNAseq to profile transcriptomes in bone marrow-derived macrophages from Nelfb KO(Nelfbfl/fl, Lyz2-Cre) and WT(Nelfb+/+,Lyz2-Cre) mice

We performed the transcriptomic analysis of RNA-seq of BMDMs generated from WT and Nelfb KO in conditions of unstimuated and LPS stimualtion to identify gene expression changes. Overall design: BMDMs from WT and KO were left untreated or stimulated with LPS for indicated time. RNA was extracted, polyA enriched and prepared for sequencing libraries.