Supplementary data from: Critical role of LdZIP7 in enhancing cadmium tolerance of Lymantria dispar larvae: Functional identification at both the individual and cellular levels

Heavy metals are prevalent environmental pollutants in habitats of phytophagous insects. This study investigates the tolerance of Lymantria dispar larvae to cadmium (Cd) and the associated mechanisms involving the ZIP family. Cd stress reduced larval body weight and extended the development duration without causing significant mortality. A significantly up-regulated expression of apoptosis genes Caspase-1, Caspase-3, and Caspase-7 was observed in Cd-exposed larvae. In Cd-exposed larvae, expression of the apoptosis-inhibitory factor Bcl-2 in the mitochondrial pathway decreased, while apoptosis-inducing factors Bax and AIFM increased. Cd stress markedly elevated the expression of CHOP and Bip, key genes in the endoplasmic reticulum stress pathway. Among the ZIP family genes, LdZIP7 showed the highest up-regulation in response to Cd treatment. Silencing LdZIP7 intensified the negative impacts of Cd stress on L. dispar larvae and significantly reduced the tolerance of L...., , # Critical role of LdZIP7 in enhancing cadmium tolerance of Lymantria dispar larvae: Functional identification at both the individual and cellular levels: Open Source Data

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We have submitted our raw data (**Data.xls**) and a folder of unmodified fluorescence micrograph images (**Figure**).

## Variable Descriptions

### Data.xls

* *C*: control group
* *LCd*: Low Cd concentration treatment group
* *HCd*: High Cd concentration treatment group
* *Cd+LdZIP7*: Sf9 cells transfected with the recombinant plasmid pAC5.1b-EGFP-2/LdZIP7 served as the experimental subjects
* *Cd+EGFP*: Untreated Sf9 cells and those transfected with EGFP were utilized as negative controls
* *Figure 1A*: The weight of the larvae at different Cd concentrations (g)
* *Figure 1B*: The developmental duration of the larvae under different Cd concentrations (h)
* *Figure 1C*: The mortality rate of the larvae under different Cd concentrations (%)
* *Figure 2*: The expression level of the ZIP gene family genes in the ...,