16S rDNA isolated and amplified from stools and colonic mucosal biopsies of patients with antibiotic associated apoptotic enterocolitis.. 16S rDNA in AAA-enterocolitis

Antibiotic therapy accounts as a major risk factor for the development of diarrhea and colitis. The presented work reports of three cases of intractable high-volume diarrhea associated with combined antibiotic and steroid therapy in critically ill patients. The patients showed severe enterocolitis, associated with epithelial mass-apoptosis resembling acute graft-versus-host-disease (GvHD) and therefore termed antibiotic associated apoptotic enterocolitis (AAA-enterocolitis). Using next-generation pyrosequencing techniques, we investigated microbial communities from stools and colonic mucosal biopsies in patients with AAA-enterocolitis and subsequent effects of fecal microbial transplants (FMT), based on 16S rDNA.The cases with AAA-enterocolitis showed severe depletion of the endogenous microbiota characterized by a significant reduction of richness, diversity and microbial load. Sample Description (lines separated by |): (SampleID) (BarcodeSequence) (sff-file) (Patient) (category) | (B stool D2) (TGTACTACTC) (1.4.sff) (B) (AAA enterocolitis) | (B mucosa D24) (TCTACGTAGC) (1.4.sff) (B) (AAA enterocolitis) | (B stool D24) (ACGACTACAG) (1.4.sff) (B) (AAA enterocolitis) | (C mucosa D33) (ACGACTACAG) (1.3.sff) (C) (Probiotic supplementation) | (C stool D33) (ATATCGCGAG) (1.4.sff) (C) (Probiotic supplementation) | (C mucosa D46) (TACGAGTATG) (1.3.sff) (C) (Probiotic supplementation) | (C stool D46) (CTCGCGTGTC) (1.4.sff) (C) (Probiotic supplementation) | (C mucosa D72) (TCGTCGCTCG) (1.3.sff) (C) (AAA enterocolitis) | (C stool D72) (TAGTATCAGC) (1.4.sff) (C) (AAA enterocolitis) | (C stool D73) (TGATACGTCT) (1.4.sff) (C) (Early post FMT) | (C stool D75) (CATAGTAGTG) (1.4.sff) (C) (Early post FMT) | (C stool 77) (CGAGAGATAC) (1.4.sff) (C) (Early post FMT) | (C mucosa D79) (ACGCGAGTAT) (1.3.sff) (C) (Early post FMT) | (C stool D79) (ATACGACGTA) (1.4.sff) (C) (Early post FMT) | (C stool D93) (CGTAGACTAG) (1.2.sff) (C) (Late post FMT) | (C mucosa D101) (ACTGTACAGT) (1.3.sff) (C) (Late post FMT) | (C mucosa D135) (AGACTATACT) (1.3.sff) (C) (Late post FMT) | (C stool D135) (TCACGTACTA) (1.4.sff) (C) (Late post FMT) | (C stool D170) (CGTCTAGTAC) (1.4.sff) (C) (Late post FMT) | (C transplant) (AGCACTGTAG) (1.4.sff) () () | (C donor stool) (AGACGCACTC) (1.4.sff) (Healthy control) (Healthy control) | (Control_stool_1) (TGATACGTCT) (1.2.sff) (Healthy control) (Healthy control) | (Control_stool_2) (TCTACGTAGC) (1.2.sff) (Healthy control) (Healthy control) | (Control_stool_3) (CGTCTAGTAC) (1.3.sff) (Healthy control) (Healthy control)