CUT-tag analysis of C1qbp sufficient and deficient CD8+ T cells

This study is aimed to Investigate whether C1qbp deficiency affects the histone modification during the differentiation stage of CD8+ T cells. Transferred WT and C1qbp KO P14 cells sorted from the spleen of donor mice on day 5 post-infection with LCMV Armstrong were collected and treated for CUT-tag. CUT-tag-Seq analysis provided evidence of histone modification changes between WT and C1qbp KO CD8+ T cells. Our study shows that on day 5 post-infection with LCMV Armstrong, along with mRNA expressing profiling, the CUT-tag-seq exhibits obvious changes in H3K27me3 and H3K27Ac modification of indicated genes of WT and C1qbp KO CD8+ T cells. Overall design: C1qbp sufficient and deficient P14 cells were transferred into donor mice and sorted from the spleen of donor mice on day 5 post-infection with LCMV Armstrong. Peaks bound by H3K27ac and H3K27me3 were obtained using CUT-tag.