Impact of sustained TGFβ-receptor inhibition on chromatin accessibility and gene expression in cultured endometrial MSC [RNA-Seq]

Endometrial mesenchymal stem cells (eMSC) drive the extraordinary regenerative capacity of the human endometrium. Clinical application of eMSC for therapeutic purposes is hampered by spontaneous differentiation and cellular senescence upon large-scale expansion in vitro. A83-01, a selective TGFbeta receptor inhibitor, promotes pharmacological expansion of eMSC in culture by blocking differentiation and senescence. We subjected primary eMSC treated with A83-01 to RNA sequencing (RNA-seq) to map the underlying gene expression changes. RNA-seq analysis demonstrated that sustained TGFbeta-R inhibition results in differential expression of 1,463 genes. We subjected A83-01 treated and untreated human primary eMSC to RNA sequencing (RNA-seq) to map the underlying gene expression changes.