Frequency of splenic B, T, NK, and NKT cell subsets in untreated or MCMV-infected WT or TLR9−/− male and female mice.

WT and TLR9−/− male and female mice were left uninfected or infected i.p. with 1,2×105 PFU of MCMV. After, 36 h spleens were harvested, total splenocytes were isolated, stained for B220, CD19, CD3, CD4, CD8, or NK1.1 and analyzed by flow cytometry. The frequency of B cells (CD19+B220+), CD4 (CD3+CD4+) and CD8 (CD3+CD8+) T cells, and NK (B220−NK1.1+CD3−), and NKT (B220−NK1.1+CD3−) cells was determined after gating among live cells. All values denote percentages (mean ± SD) of three mice per group, and are representative of two or three independent experiments.