Bio-oil aqueous phase as a laccase inducer in Pleurotus ostreatus: laccase properties and applications, Iowa, USA 2021-2023

This dataset documents the experimental conditions, protocols, and measurements generated during the investigation of bio-oil aqueous phase (AP) as an inducer for laccase enzyme production by Pleurotus ostreatus using prairie biomass as a substrate. The data include detailed descriptions of AP preparation from corn stover pyrolysis, chemical characterization of AP and prairie biomass, and the design of two-stage solid-state fermentation experiments with variables such as AP and copper concentrations, fermentation times, and environmental parameters (pH, temperature). The dataset also contains raw and processed measurements of laccase activity, protein concentration, reducing capacity, and other relevant biochemical properties across multiple experimental runs. In addition to fermentation and enzyme production data, the dataset provides information on downstream application assays. These include quantitative data on dye decolorization (crystal violet, coomassie blue, methyl orange), saccharification yields from prairie biomass pretreated with laccase, and antibiotic detoxification assays using tetracycline.