Genomic analyses of Klebsiella pneumoniae isolates from Hospital for Tropical Diseases in Ho Chi Minh City, 2010-2014 and the circulation of blaNDM-1 carrying plasmids

Klebsiella pneumoniae is becoming a leading pathogen causing disease in human. Previously, K. pneumoniae was thought of as an opportunistic pathogen. However, otherwise healthy individuals are now commonly presenting with K. pneumoniae blood infections, which can result in serious diseases such as liver abscesses and septic shock. K. pneumoniae is an important reservoir for antimicrobial resistance (AMR) genes. The eponymous Klebsiella Pneumoniae Carbapenemase (KPC) and New Delhi Metallo-beta-lactamase-1 (NDM-1), were first identified in K. pneumoniae and firstly described in Viet Nam in 2010 but there is little known about this pathogen. The genomic analysis in this study defined that lineage of KpI was the most common lineage in the Hospital for Tropical Disease, Viet Nam (91.5%). The sequence type of ST23 was determined as the most prevalent (18.5%) as highly adaptability by acquiring several virulence factors. There were three different strains carrying blaNDM-1 to against carbapenems. It was located on the same gene cassette and on the distinctive plasmid backbones. Particular insight into molecular mechanisms will help to raise public awareness of the disease and to have an improved infection control strategy.