Multi-omics profiling visualizes dynamics of cardiac development and functions

The function and phenotype transitions of heart may be specific to one or other chamber. To provide the omics data sets from chambers separately, we conducted spatial transcriptomics of E10.5 hearts to identify unique gene profiles that correspond to distinct anatomical regions in early cardiac development. We obtained curated expression data consisting of 6,798 genes across 332 individual capture spots. We then performed differential gene expression (DGE) analysis between anatomical regions and gene set enrichment analysis of each cluster’s upregulated genes to characterize identified clusters in early cardiogenesis. 10X Visium was used to create a transcriptomic atlas of mouse embryonic day 10.5 heart.