RNA-seq in HUVECs upon DDX24 knockdown

DEAD-box (DDX) RNA helicases play crucial roles in gene regulation by interacting with RNAs and influencing RNA fate and function. Previously, we have associated DDX24 with vascular development, but its precise role in RNA metabolism remains unclear. In this study, we demonstrate that DDX24 modulated endothelial cell functions by binding to and regulating mRNAs crucial for vascular morphogenesis and angiogenesis. These results establish a link between DDX24-dependent regulation of mRNA stability and endothelial cell function, providing novel therapeutic targets for angiogenesis. Overall design: To investigate the role of DDX24 in vascular endothelial cells, RNA-seq of HUVEC with DDX24 knockdown by siRNA was performed. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different processed cells.Comparative gene expression profiling analysis of RNA-seq data for HUVECs(siNC) and its derivative (siDDX24).