Oenothera glazioviana root LC-MS

Proteomic studies were performed to identify the proteins involved in copper (Cu) stress responses of Oenothera glazioviana seedlings. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth, and the level of lipid peroxidation in the roots was markedly increased. Compared to shoot, Cu absorbed by Oenothera glazioviana more easily accumulation in root. Label-free proteomic analysis indicated 58 differentially abundance proteins (DAPs) of 3149 total proteins in the roots of O. glazioviana seedlings, of which, 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu caused alleviation of stress symptoms. Overall, this study provides new understanding into the molecular mechanisms at protein level of Cu response in candidate plants contributed to soil properties.