Single-cell RNA sequencing of circulating mock-electroporated CD2 wild-type and CD2-knockout 41BBzeta CART19 cells

Single-cell gene expression of 4-1BBzeta CART19 cells in both the peripheral blood of OCI-Ly18 DLBCL-bearing mice. Cells were captured and processed using 10X 5' v2 Chemistry GEX Overall design: CART19 cells generated from a healthy human donor were administered to mice bearing OCI-Ly18 tumors. Briefly, on day -10, 5 × 10^6 luciferase-expressing OCI-Ly18 cells were implanted subcutaneously into NSG mice. On day 0, tumor-bearing mice received an infusion of 4 × 10^6 CART19 cells that were either CD2 wild-type (n = 8, mock electroporated) or CD2 knockout (n = 6). On day 16, peripheral blood was collected via the retro-orbital sinus. Human T cells from individual mice were subsequently barcoded, pooled according to experimental group (WT or CD2 KO), and isolated by FACS based on CD3?CD2?CD5? expression.