Gametocyte, oocyst and sporozoite development of Δp52 parasites.

aExflagellation (Exfl) of male gametocytes was determined in small samples from the cultures by counting exflagellation centres under the light-microscope in 25 homogeneous fields of rbc at a 40× magnification. A mean of 2–10 per field is scored as +; >10 as ++ and less then 2 as +/−.bOocyst production is the median of the oocysts counted at day 7 after mosquito feeding and IQR is the inter quartile range. No significant difference exist between mutant and wild-type parasites (Wilcoxin rank-sum test; p = 0.13 for Δp52-1 and p = 0.5 for Δp52-2).