Gene expression profile at single cell level of human melanoma cell lines, normal human melanocytes and human nevus cells.

Melanomas are considered highly heterogenous in transcriptional state. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of melanoma cells as compared to primary melanocytes and nevus melanocytes. Sample 1 contain KIT postitive melanocytes sequenced directly ex vivo from human nevi. Sample 2 contains different human melanoma cell lines and primary human melanocytes grown in vitro. Overall design: Melanoma cell lines were grown in RPMI media supplemented with 10% FBS. NHMs were grown in 254 media supplemented with HGMS. Nevus cells were obtained by excising nevi and sorting for KIT positive cells using the protocol in PMID: 34475532