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TMT-based quantitative MS analysis of proteome labeled by NO-responsive reaction with NOAR-2

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https://www.omicsdi.org/dataset/jpost/PXD012310
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RAW264.7 cells were treated by LPS and IFN-gamma for 20 h. The LPS/IFN treated or nontreated cells were labeled by NOAR-2 for 1 h. Cells were lysed by NP-40 buffer. Labeled proteins were purified by immunopecipitation using anti-fluorescein, then digested by trypsin. Digested peptides were labeled by TMTsixplex isobaric labeling reagents, followed by mass spectrometry measurement.
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2020-01-12
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