Interaction of XopJ with RPT6 in Yeast Two-Hybrid Assays.

XopJ fused to the GAL4 DNA binding domain (BD) was expressed in combination with RPT6 fused to the GAL4 activation domain (AD) in yeast strain Y190. Cells were grown on selective media before a LacZ filter assay was performed. pSV40/p53 served as positive control while the empty AD vector served as negative control. NtRPT6, N. tabacum RPT6; AtRPT6a, A. thaliana RPT6 isoform a; AtRPT6b, A. thaliana RPT6 isoform b; ScRPT6, S. cerevisiae RPT6. – LT, yeast growth on medium without Leu and Trp. – HLT, yeast growth on medium lacking His, Leu, and Trp, indicating expression of the HIS3 reporter gene. LacZ, activity of the lacZ reporter gene.