Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and FAD4T thyroid Transcriptomes,Young and Aging thyroid Transcriptomes

A recent study showed thyroid function of both humans and animals decrease as they age. Muchmore, the incidence of thyroid dysfunction was also higher in patients with Alzheimer's disease than in normal people. The goals of this study were to study the molecular mechanism of thyroid dysfunction in FAD4T and aging by transcriptome profiling (RNA-seq). Therefore, we collected the thyroids from 2 month-old wild-type (WT) and 18-month natural aging mouse, 2-month-old WT and 2-month FAD4T mouse, 8-month-old WT and 8-month FAD4T mouse, and then performed RNA sequencing. Overall design: 2-month FAD4T (n=3), 2-month WT (n=3), 8-month FAD4T (n=3), and 8-month WT (n=3) female mice and the same number male mice as above, and 2-month WT (n=3), 18-maonth WT (n=3) male mice